1. Check and clean the injector assembly:
Regular cleaning of the injector assembly ensures that sample carryover is minimized, maintaining data accuracy.
2. Inspect and clean the detector flow cell:
A clean flow cell ensures accurate detection of compounds, preventing signal interference.
3. Verify the integrity of the column and replace if necessary:
Damaged or degraded columns can lead to poor separations and inaccurate results, necessitating replacement.
4. Check and clean the sample loop:
A clean sample loop reduces the risk of contamination and ensures consistent injections.
5. Inspect and clean the pump seals and pistons.:
Proper maintenance of pump components is essential for precise and reliable flow rates.
6. Examine the tubing and replace any worn or damaged sections:
Damaged tubing can cause leaks and disrupt the flow path, affecting chromatographic performance.
7. Verify the performance of the autosampler, including its syringe and sample needle:
A well-functioning autosampler is crucial for accurate and reproducible injections.
8. Check the system for leaks, both in the high-pressure and low-pressure areas:
Identifying and addressing leaks promptly prevents damage to the instrument and maintains system integrity.
9. Inspect and clean the solvent reservoir:
Clean solvent reservoirs to prevent contamination of mobile phases and maintain system cleanliness.
10. Calibrate and validate the system regularly to maintain accuracy:
Regular calibration and validation ensure that the HPLC system consistently delivers reliable results.
